2023 Season
| Team | Basketball | Softball |
| Community Mennonite Church | Yes | |
| Community Mennonite Church / Harrisonburg Mennonite Church | Yes (D1) | |
| Covenant Presbyterian Church | Yes (D1) | |
| Dayton Mennonite Church I | Yes | Yes (D1) |
| Dayton Mennonite Church II | Yes (D2) | |
| Divine Unity Community Church | Yes (D1) | |
| East Side Church | Yes | |
| East Side Church / Park View Mennonite Church | Yes (D2) | |
| Faith Community Church | Yes | Yes (D2) |
| First Russian Baptist Church | Yes | |
| Gospel Hill Mennonite Church | Yes (D2) | |
| Harrisonburg Baptist Church | Yes (D2) | |
| Harrisonburg First Church of the Nazarene | Yes (D2) | |
| Mountain View Fellowship | Yes | |
| New Beginnings Church | Yes | Yes (D2) |
| Park View Mennonite Church | Yes | |
| Pleasant Valley Church of the Brethren | Yes | |
| Valley View Mennonite Church | Yes (D2) | |
| Weavers Mennonite Church | Yes | Yes (D1) |
| West Side Baptist Church | Yes (D2) | |
| Zion Mennonite Church / Ridgeway Mennonite Church | Yes (D1) |